Project Description:

PT. Paragon Technology and Innovation, one of the leading cosmetic manufacturing company in Indonesia that produces cosmetic brand such as Wardah, Emina, Make Over and others, has proposed to do a collaboration project for in vitro evaluation of one of their skincare products to analyze their capability in protecting the skin from blue-light and pollutant. Evaluating the protective ability towards pollutants will be based on the principle of measuring and comparing the viability of keratinocytes between groups that are treated and untreated with products after insult treatment with a pollutant model in the form of free radicals from oxygen. In vitro results of this study will give the basis of cosmetic manufacturers to claim the protective efficacy of their product based on obtained in vitro results. Scope of the project will include protective capability measurement of product towards keratinocytes cell culture which will be treated and induced with pollutant or blue-light insult model. The blue-light induction will be performed through exposure of light using light source with wavelength within blue-light range (400-500 nm, peak 450) while a model of pollutant induction will be performed through peroxide insult as a reactive oxygen species (ROS). The cell viability will be compared between groups treated and not treated with products to analyze their protective capability.